BCL2L2 Polyclonal antibody

BCL2L2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16026-1-AP

Synonyms

Apoptosis regulator Bcl W, BCL2L2, KIAA0271



Tested Applications

Positive WB detected inHL-60 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

16026-1-AP targets BCL2L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L2 fusion protein Ag8863
Full Name BCL2-like 2
Calculated molecular weight 193 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC021198
Gene symbol BCL2L2
Gene ID (NCBI) 599
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCL2L2 antibody 16026-1-APDownload protocol
IHC protocol for BCL2L2 antibody 16026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Peptides Isolated from Yak Milk Residue Exert Antioxidant Effects through Nrf2 Signal Pathway.

Authors - Feiyan Yang
humanWB

Tumour Biol

MicroRNA-15a induces cell apoptosis and inhibits metastasis by targeting BCL2L2 in non-small cell lung cancer.

Authors - Tian Yang
ratWB

Eur J Pharmacol

GSK-3β-mediated activation of NLRP3 inflammasome leads to pyroptosis and apoptosis of rat cardiomyocytes and fibroblasts.

Authors - Shu-Hui Wang
humanWB

PLoS One

Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.

Authors - Yu Guohua G
humanWB

iScience

HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6.

Authors - Menghui Yin
humanWB

Cancers (Basel)

Gemcitabine Cooperates with Everolimus to Inhibit the Growth of and Sensitize Malignant Meningioma Cells to Apoptosis Induced by Navitoclax, an Inhibitor of Anti-Apoptotic BCL-2 Family Proteins.

Authors - Masahiro Yamamoto