CoraLite® Plus 488-conjugated BCL3 Polyclonal antibody

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  IF Staining of HepG2 using CL488-23959

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 BCL3 antibody (CL488-23959) at dilution of 1:200.

• Featured Product
• KD/KO Validated

BCL3 Polyclonal Antibody for IF/ICC

Cat No. CL488-23959

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37, Proto-oncogene BCL3

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-23959 targets BCL3 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL3 fusion protein Ag21040
• Full Name: B-cell CLL/lymphoma 3
• Calculated Molecular Weight: 446 aa, 47 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC064993
• Gene Symbol: BCL3
• Gene ID (NCBI): 602
• RRID: AB_3084077
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20749
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248).