BCL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 23959-1-AP

Synonyms

B cell CLL/lymphoma 3, B cell lymphoma 3 protein, BCL3, D19S37, Proto oncogene BCL3

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Validation Data Gallery

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  WB analysis of HeLa using 23959-1-AP

  WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells.

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  WB analysis of HEK-293 using 23959-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23959-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23959-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23959-1-AP

  IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug.

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  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 23959-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 23959-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lymphoma tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

23959-1-AP targets BCL3 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL3 fusion protein Ag21040
• Full Name: B-cell CLL/lymphoma 3
• Calculated Molecular Weight: 446 aa, 47 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC064993
• Gene Symbol: BCL3
• Gene ID (NCBI): 602
• RRID: AB_2879375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	Nucleic Acids Res; SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators.; Authors - Xiaojun Ma
human	WB,IP	Mol Cancer Ther; The Discovery of a Novel Antimetastatic Bcl3 Inhibitor.; Authors - Jitka Soukupová
human	WB	Carcinogenesis; Bcl-3 promotes multi-modal tumour cell migration via NF-κB1 mediated regulation of Cdc42.; Authors - Daniel J Turnham ; KD Validated
human	WB,chIP	J Biol Chem; The proto-oncogene Bcl3 induces immune checkpoint PD-L1 expression, mediating proliferation of ovarian cancer cells.; Authors - Yue Zou ; KD Validated
human	WB	Int J Oncol; BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer.; Authors - Tracey Jane Collard

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Bone Marrow MSCs

FH

Ewelina (Verified Customer) (07-03-2019)

This is the only antibody for Western Blot, that worked for me for the detection of endogenous BCL3 in human lymphoma cell lines. It works perfectly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human lymphoma cell lines WT and KO