BCOR Polyclonal antibody

BCOR Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12107-1-AP

Synonyms

ANOP2, BCOR, KIAA1575, MAA2, MCOPS2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12107-1-AP targets BCOR in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCOR fusion protein Ag2752
Full Name BCL6 co-repressor
Calculated Molecular Weight 1703 aa, 186 kDa
Observed Molecular Weight 186 kDa
GenBank Accession NumberBC009675
Gene Symbol BCOR
Gene ID (NCBI) 54880
RRIDAB_2290335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem

Protocols

Product Specific Protocols
WB protocol for BCOR antibody 12107-1-APDownload protocol
IHC protocol for BCOR antibody 12107-1-APDownload protocol
IP protocol for BCOR antibody 12107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB, IHC

Nat Genet

Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney.

Authors - Hitomi Ueno-Yokohata
humanWB

Nucleic Acids Res

Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.

Authors - Dongxue Su
mouseWB

Int J Mol Sci

BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.

Authors - Satoshi Nakata
mouseWB

Sci Rep

Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells.

Authors - Yun Yan

Cell Prolif

Secreted frizzled-related protein 2 promotes the osteo/odontogenic differentiation and paracrine potentials of stem cells from apical papilla under inflammation and hypoxia conditions.

Authors - Haoqing Yang
humanIHC

J Clin Pathol

Novel exon-exon breakpoint in CIC-DUX4 fusion sarcoma identified by anchored multiplex PCR (Archer FusionPlex Sarcoma Panel).

Authors - Benjamin Nathanael Loke

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sid (Verified Customer) (05-13-2019)

Works well for mouse hematopoetic cells, some strong non specific bands ~ 150 kD and ~100 kD, however can be reasonably resolved on 4-15% gel.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse hematopoetic