BDNF Monoclonal antibody
BDNF Monoclonal Antibody for IF, WB, ELISA
Host / Isotype
Mouse / IgG2a
human and More (1)
WB, IHC, IF, FC, ELISA
Cat no : 66292-1-Ig
|Positive WB detected in||U-251 cells, C6 cells, fetal human brain tissue|
|Positive IF detected in||U-251 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|WB||See 19 publications below|
|IHC||See 3 publications below|
|IF||See 4 publications below|
|FC||See 1 publications below|
66292-1-Ig targets BDNF in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||BDNF fusion protein Ag11329|
|Full Name||brain-derived neurotrophic factor|
|Calculated molecular weight||247 aa, 28 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC029795|
|Gene ID (NCBI)||627|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
|Product Specific Protocols|
|WB protocol for BDNF antibody 66292-1-Ig||Download protocol|
|IF protocol for BDNF antibody 66292-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.
Perfluorooctane sulfonates induces neurobehavioral changes and increases dopamine neurotransmitter levels in zebrafish larvae.
GABA-mediated activated microglia induce neuroinflammation in the hippocampus of mice following cold exposure through the NLRP3 inflammasome and NF-κB signaling pathways.
Front Cell Neurosci
Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.
Effect of Qingyangshen glycosides on social defeat mice model.
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Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.