|Positive WB detected in||U-251 cells, C6 cells, fetal human brain tissue|
|Positive IF detected in||U-251 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66292-1-Ig targets BDNF in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||BDNF fusion protein Ag11329|
|Full Name||brain-derived neurotrophic factor|
|Calculated molecular weight||247 aa, 28 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC029795|
|Gene ID (NCBI)||627|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).
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