BDNF Monoclonal antibody

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  WB analysis of U-251 using 66292-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66292-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of PC-12 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

BDNF Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66292-1-Ig

Clone No.1E5C5

Publications(41)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

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Tested Applications

• Positive WB detected in: U-251 cells, fetal human brain tissue
• Positive IF/ICC detected in: U-251 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 32 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• FC: See 1 publications below

Product Information

66292-1-Ig targets BDNF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, zebrafish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BDNF fusion protein Ag11329
• Full Name: brain-derived neurotrophic factor
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC029795
• Gene Symbol: BDNF
• Gene ID (NCBI): 627
• RRID: AB_2881675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23560
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

Species	Application	Title
human	IHC	Cancer Lett; Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.; Authors - Yansong Liu
	IF	Int J Mol Sci; Methotrexate Neurotoxicity Is Related to Epigenetic Modification of the Myelination Process.; Authors - Yu-Chieh Chen
	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun
zebrafish	WB	Chemosphere; Perfluorooctane sulfonates induces neurobehavioral changes and increases dopamine neurotransmitter levels in zebrafish larvae.; Authors - Luyin Wu
	WB	Int Immunopharmacol; Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model; Authors - Xin-Mei Xie
	WB	Exp Neurol; DPP-4 inhibition by linagliptin ameliorates age-related mild cognitive impairment by regulating microglia polarization in mice; Authors - Fen Zhuge