CoraLite®594-conjugated BDNF Monoclonal antibody

BDNF Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E5C5

Cat no : CL594-66292

Synonyms

Abrineurin, BDNF



Tested Applications

Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL594-66292 targets BDNF in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BDNF fusion protein Ag11329
Full Name brain-derived neurotrophic factor
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC029795
Gene symbol BDNF
Gene ID (NCBI) 627
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Protocols

Product Specific Protocols
IF protocol for CL594 BDNF antibody CL594-66292Download protocol
Standard Protocols
Click here to view our Standard Protocols