CoraLite® Plus 488-conjugated BDNF Monoclonal antibody
BDNF Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2a
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-66292
|Positive IF detected in||PC-12 cells|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
CL488-66292 targets BDNF in IF applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||BDNF fusion protein Ag11329|
|Full Name||brain-derived neurotrophic factor|
|Calculated molecular weight||247 aa, 28 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC029795|
|Gene ID (NCBI)||627|
|Conjugate||CoraLite® Plus 488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||493 nm / 522 nm|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1) What is BDNF?
BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.
2) FAQs for BDNF
A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).