BEND3 Polyclonal antibody, PBS Only

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  WB analysis using 23101-1-AP (same clone as 23101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 23101-1-AP (same clone as 23101-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23101-1-AP (same clone as 23101-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

BEND3 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 23101-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

BEN domain containing 3, BEN domain-containing protein 3, KIAA1553, RP11 59I9.2

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Product Information

23101-1-PBS targets BEND3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19137

  Product name: Recombinant human BEND3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 493-828 aa of BC131628

  Sequence: PPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFDKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYSCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLTSYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSPYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK

• Full Name: BEN domain containing 3
• Calculated Molecular Weight: 828 aa, 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC131628
• Gene Symbol: BEND3
• Gene ID (NCBI): 57673
• RRID: AB_2879210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5T5X7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)