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TGFBI / BIGH3 Monoclonal antibody

TGFBI / BIGH3 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human and More (1)







Cat no : 60007-1-Ig


Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

Tested Applications

Positive WB detected inhuman kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human skin cancer tissue, human colon cancer tissue, human placenta tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60007-1-Ig targets TGFBI / BIGH3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TGFBI / BIGH3 fusion protein Ag0241
Full Name transforming growth factor, beta-induced, 68kDa
Calculated Molecular Weight 683 aa, 75 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC000097
Gene Symbol TGFBI
Gene ID (NCBI) 7045
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).


Product Specific Protocols
WB protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
IHC protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
IF protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
IP protocol for TGFBI / BIGH3 antibody 60007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Res

TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.

Authors - Laura S M Lecker

Cell Rep

Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury

Authors - Yiheng Mao

Int J Cancer

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

Authors - Ween Miranda P MP

Cell Mol Life Sci

TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.

Authors - Irigoyen M M

Front Oncol

Identification of Hub Genes Associated With Sensitivity of 5-Fluorouracil Based Chemotherapy for Colorectal Cancer by Integrated Bioinformatics Analysis.

Authors - Ya Wang


BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Authors - Tianhong Pan
  • KD Validated