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CoraLite®488-conjugated TGFBI / BIGH3 Monoclonal antibody

TGFBI / BIGH3 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3E11D11

Cat no : CL488-60007

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3



Tested Applications

Positive IF detected inhuman colon cancer tissue
Positive FC detected inY79 cells
For other applications, we recommend the unconjugated version of this antibody, 60007-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60007 targets TGFBI / BIGH3 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TGFBI / BIGH3 fusion protein Ag0241
Full Name transforming growth factor, beta-induced, 68kDa
Calculated molecular weight 683 aa, 75 kDa
GenBank accession numberBC000097
Gene symbol TGFBI
Gene ID (NCBI) 7045
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Protocols

Product Specific Protocols
IF protocol for CL488 TGFBI / BIGH3 antibody CL488-60007Download protocol
Standard Protocols
Click here to view our Standard Protocols