CoraLite®594-conjugated TGFBI / BIGH3 Monoclonal antibody

• Featured Product
• KD/KO Validated

TGFBI / BIGH3 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E11D11

Cat no : CL594-60007

Synonyms

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

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Validation Data Gallery

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  FC experiment of Y79 using CL594-60007

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TGFBI / BIGH3 (CL594-60007, Clone:3E11D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human colon cancer tissue
• Positive FC detected in: Y79 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60007 targets TGFBI / BIGH3 in IF, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TGFBI / BIGH3 fusion protein Ag0241
• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 683 aa, 75 kDa
• GenBank Accession Number: BC000097
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• RRID: AB_2919887
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C