Validation Data Gallery
|Positive WB detected in
|mouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, mouse testis tissue
|Positive IHC detected in
|human testis tissue, mouse pancreas tissue, mouse testis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10022-1-AP targets cIAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, zebrafish
|Host / Isotype
|Rabbit / IgG
|cIAP1 fusion protein Ag15203
|baculoviral IAP repeat-containing 2
|Calculated molecular weight
|618 aa, 70 kDa
|Observed molecular weight
|70 kDa, 55-60 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.
Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.
Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.
A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.
Acta Pharm Sin B
A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.
Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.
Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.