Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue, mouse pancreas tissue, mouse testis tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10022-1-AP targets cIAP1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15203 Product name: Recombinant human cIAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC016174 Sequence: MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQG Predict reactive species |
| Full Name | baculoviral IAP repeat-containing 2 |
| Calculated Molecular Weight | 618 aa, 70 kDa |
| Observed Molecular Weight | 70 kDa, 55-60 kDa |
| GenBank Accession Number | BC016174 |
| Gene Symbol | cIAP1 |
| Gene ID (NCBI) | 329 |
| RRID | AB_2063790 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13490 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| IF protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| IHC protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| WB protocol for cIAP1 antibody 10022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The cIAP1 (BIRC2) antibody (Cat# 10022-1-AP) has 27 citations spanning journals including Nature Genetics, Molecular Cell, Developmental Cell, Journal of Biological Chemistry, Journal of Immunology, Cell Death & Disease, Oncotarget, and Frontiers in Pharmacology. Research fields center on NF-κB signaling, apoptosis and IAP-mediated cell death, ubiquitination, and cancer biology (glioblastoma, nasopharyngeal, gastric, lung, and medulloblastoma), plus inflammation, mitophagy, and vascular homeostasis.
| Species | Application | Title |
|---|---|---|
Nat Genet Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.
| ||
Mol Cell Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB. | ||
Mol Cell A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.
| ||
Acta Pharm Sin B A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein. | ||
J Exp Clin Cancer Res TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein | ||
Cell Death Dis BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma |
Reviews
What customers say
The single available review rates this antibody 5 stars. The reviewer used it for immunoprecipitation at a 1:1000 dilution in A549 cells and described the performance as "Excellent." Overall, the feedback is positive, indicating reliable results in IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (09-23-2024) | Excellent
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