cIAP2 Polyclonal antibody

cIAP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24304-1-AP

Synonyms

AIP1, API2, Apoptosis inhibitor 2, BIRC3, C IAP2, CIAP2, HAIP1, hIAP 1, HIAP1, IAP 1, IAP homolog C, IAP1, MALT2, MIHC, RING finger protein 49, RNF49



Tested Applications

Positive WB detected inDaudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells
Positive IP detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24304-1-AP is cIAP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cIAP2 fusion protein Ag20143
Full Name baculoviral IAP repeat-containing 3
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC027485
Gene symbol BIRC3
Gene ID (NCBI) 330
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for cIAP2 antibody 24304-1-APDownload protocol
IHC protocol for cIAP2 antibody 24304-1-APDownload protocol
IP protocol for cIAP2 antibody 24304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Death Dis

The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.

Authors - Tian Xiang
humanWB

Cell Death Dis

Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.

Authors - Jingru Huangfu
humanWB

Mol Biol Rep

Chlorogenic acid inhibits proliferation in human hepatoma cells by suppressing noncanonical NF-κB signaling pathway and triggering mitochondrial apoptosis.

Authors - Yanfei Jiang
humanWB

Biochem Biophys Res Commun

PMID: 25767076

Authors - Ping Wu
humanWB

Cancer Biol Ther

Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.

Authors - Liu Qin Q
humanIHC

Cancer Med

Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.

Authors - Shu-Mei Chen