cIAP2 Polyclonal antibody

cIAP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24304-1-AP

Synonyms

AIP1, API2, Apoptosis inhibitor 2, BIRC3, C IAP2, CIAP2, HAIP1, hIAP 1, HIAP1, IAP 1, IAP homolog C, IAP1, MALT2, MIHC, RING finger protein 49, RNF49



Tested Applications

Positive WB detected inDaudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells
Positive IP detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

24304-1-AP targets cIAP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen cIAP2 fusion protein Ag20143
Full Name baculoviral IAP repeat-containing 3
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC027485
Gene symbol BIRC3
Gene ID (NCBI) 330
RRIDAB_2879485
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for cIAP2 antibody 24304-1-APDownload protocol
IHC protocol for cIAP2 antibody 24304-1-APDownload protocol
IP protocol for cIAP2 antibody 24304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

Authors - Maria Zachari
humanWB

Oncotarget

The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.

Authors - Tian-Hao Liu
humanIHC

Front Oncol

Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.

Authors - Jinguo Zhang
human,mouseWB

Biomed Pharmacother

Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.

Authors - Lei Wang
humanIHC

Cancer Med

Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.

Authors - Shu-Mei Chen
humanWB

Cancer Biol Ther

Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.

Authors - Liu Qin Q