cIAP2 Polyclonal antibody

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  WB analysis using 24304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 24304-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 24304-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Daudi using 24304-1-AP

  IP result of anti-cIAP2 (IP:24304-1-AP, 4ug; Detection:24304-1-AP 1:1000) with Daudi cells lysate 2800ug.

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  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24304-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

cIAP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 24304-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

BIRC3, CIAP 2, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3

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Tested Applications

• Positive WB detected in: Daudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells
• Positive IP detected in: Daudi cells
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 5 publications below

Product Information

24304-1-AP targets cIAP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: cIAP2 fusion protein Ag20143
• Full Name: baculoviral IAP repeat-containing 3
• Calculated Molecular Weight: 604 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC027485
• Gene Symbol: cIAP2
• Gene ID (NCBI): 330
• RRID: AB_2879485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13489
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.

Publications

Species	Application	Title
human	WB	Dev Cell; Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.; Authors - Maria Zachari
human	WB	Oncotarget; The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.; Authors - Tian-Hao Liu
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
human,mouse	WB	Biomed Pharmacother; Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.; Authors - Lei Wang
human	IHC	Cancer Med; Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.; Authors - Shu-Mei Chen
human	WB	Cancer Biol Ther; Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.; Authors - Liu Qin Q