BLK Polyclonal antibody

BLK Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10510-1-AP

Synonyms

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK, p55 Blk, Tyrosine protein kinase Blk



Tested Applications

Positive WB detected inJurkat cells, Raji cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10510-1-AP targets BLK in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLK fusion protein Ag0795
Full Name B lymphoid tyrosine kinase
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007371
Gene symbol BLK
Gene ID (NCBI) 640
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Protocols

Product Specific Protocols
WB protocol for BLK antibody 10510-1-APDownload protocol
IHC protocol for BLK antibody 10510-1-APDownload protocol
IP protocol for BLK antibody 10510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols