BLK Polyclonal antibody

BLK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10510-1-AP

Synonyms

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK, p55 Blk, Tyrosine protein kinase Blk



Tested Applications

Positive WB detected inJurkat cells, Raji cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10510-1-AP targets BLK in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLK fusion protein Ag0795
Full Name B lymphoid tyrosine kinase
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC007371
Gene Symbol BLK
Gene ID (NCBI) 640
RRIDAB_2274754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Protocols

Product Specific Protocols
WB protocol for BLK antibody 10510-1-APDownload protocol
IHC protocol for BLK antibody 10510-1-APDownload protocol
IP protocol for BLK antibody 10510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin

Authors - Cefan Zhou
humanWB

Cancer Cell

Molecular targets of glucocorticoids that elucidate their therapeutic efficacy in aggressive lymphomas

Authors - Jaewoo Choi