|Positive WB detected in||human placenta tissue, HEK-293 cells|
|Western Blot (WB)||WB : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
18298-1-AP targets BMF in WB, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||Bcl2 modifying factor|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||BC069505|
|Gene ID (NCBI)||90427|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.
Characterization of an alternative BAK-binding site for BH3 peptides.
Int J Biol Sci
RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression.
Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.
BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chi (Verified Customer) (09-28-2019)
This antibody generally works well to detect BMF in western blot at a relatively high concentration.