BMF Polyclonal antibody

• Featured Product
• KD/KO Validated

BMF Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 18298-1-AP

Synonyms

FLJ00065, Bcl-2-modifying factor

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Validation Data Gallery

• 

  IP experiment of HEK-293 using 18298-1-AP

  IP result of anti-BMF (IP:18298-1-AP, 4ug; Detection:18298-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

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Tested Applications

• Positive IP detected in: HEK-293 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

18298-1-AP targets BMF in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Bcl2 modifying factor
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC069505
• Gene Symbol: BMF
• Gene ID (NCBI): 90427
• RRID: AB_2259162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Characterization of an alternative BAK-binding site for BH3 peptides.; Authors - Kaiqin Ye
human	WB	Int J Biol Sci; RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression.; Authors - Feng Xu ; KD Validated
human	WB	PLoS One; Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.; Authors - Yu Guohua G
mouse	WB	Aging Dis; BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo; Authors - Xiao Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chi (Verified Customer) (09-28-2019)

This antibody generally works well to detect BMF in western blot at a relatively high concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse embryonic fibroblasts

BMF Antibody Western Blot, validation (1:500 dilution) in mouse embryonic fibroblasts (Cat no:18298-1-AP)