BMF Polyclonal antibody

BMF Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 18298-1-AP

Synonyms

BMF



Tested Applications

Positive WB detected inhuman placenta tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

18298-1-AP targets BMF in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Bcl2 modifying factor
Calculated molecular weight 21 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC069505
Gene symbol BMF
Gene ID (NCBI) 90427
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.

Protocols

Product Specific Protocols
WB protocol for BMF antibody 18298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.

Authors - Yu Guohua G
mouseWB

Nat Commun

Characterization of an alternative BAK-binding site for BH3 peptides.

Authors - Kaiqin Ye

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chi (Verified Customer) (09-28-2019)

This antibody generally works well to detect BMF in western blot at a relatively high concentration.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse embryonic fibroblasts
BMF Antibody Western Blot, validation (1:500 dilution) in mouse embryonic fibroblasts (Cat no:18298-1-AP)