BMP6 Polyclonal antibody

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  WB analysis using 55421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55421-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens).

BMP6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55421-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

BMP-6, BMP 6, VG 1 related protein, VG 1 R, Bone Morphogenic Protein 6

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Tested Applications

• Positive WB detected in: A549 cells, mouse liver tissue, HEK-293 cells, HL-60 cells, U2OS cells
• Positive IHC detected in: human tonsillitis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

55421-1-AP targets BMP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: bone morphogenetic protein 6
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_001718
• Gene Symbol: BMP6
• Gene ID (NCBI): 654
• RRID: AB_3086450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22004
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic protein 6 (BMP6) is also named as VG-1-related protein (VG-1-R, VGR-1) and VGR, and belongs to the TGF-beta family (PMID:33878365). Growth factor of the TGF-beta superfamily plays essential roles in many developmental processes including cartilage and bone formation (PMID:31019025). BMP6 participates in iron homeostasis, inhibits invasion by increasing adhesions and cell-cell type interactions and induces angiogenesis directly on vascular endothelial cells. BMP6 is coded by a tumor suppressor gene whose subexpression is related to the development and cancer progression; during neoplastic processes, methylation is the main mechanism by which gene silencing occurs (PMID:34706618). BMP6 plays an important role in the regulation of hepcidin/HAMP expression and iron metabolism by acting as a ligand for hemojuvelin/HJV (PMID:26582087). BMP6 initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B (PMID:18070108).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Neuroimmune Pharmacol; Ghrelin Induces Ferroptosis Resistance and M2 Polarization of Microglia to Alleviate Neuroinflammation and Cognitive Impairment in Alzheimer's Disease; Authors - Yaoxue Guo
human	WB	Cell Tissue Res; Bone morphogenetic protein 6 (BMP6) antagonises experimental proliferative vitreoretinopathy established by TGF-β2 stimulation in retinal pigment epithelial cells through modulation of the p38 and JNK MAPK pathways; Authors - Xuan Liu
human,rat	IHC	J Pharmacol Sci; Icariin alleviates diabetic renal interstitial fibrosis aggravation by inhibiting miR-320a-3p targeting BMP6; Authors - Kaiwei Wang
human	WB	J Assist Reprod Genet; BMAL1 improves assisted reproductive technology outcomes in patients with polycystic ovary syndrome by targeting BMP6 and regulating ovarian granulosa cell apoptosis; Authors - Qihui Liang
mouse	WB	Geroscience; Tilorone attenuates high-fat diet-induced hepatic steatosis by enhancing BMP9-Smad1/5/8 signaling; Authors - Barnabas Horvath