BMP7 Polyclonal antibody

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  WB analysis using 12221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12221-1-AP (BMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12221-1-AP

  WB result of BMP7 antibody (12221-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMP7 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 12221-1-AP

  IP result of anti-BMP7 (IP:12221-1-AP, 4ug; Detection:12221-1-AP 1:800) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human bladder using 12221-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 12221-1-AP (BMP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12221-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BMP7 antibody (12221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

BMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12221-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

OP1, OP 1, Eptotermin alfa, Bone morphogenetic protein 7, BMP-7

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human bladder tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 8 publications below

Product Information

12221-1-AP targets BMP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMP7 fusion protein Ag2902
• Full Name: bone morphogenetic protein 7
• Calculated Molecular Weight: 431 aa, 49 kDa
• Observed Molecular Weight: 43-49 kDa
• GenBank Accession Number: BC008584
• Gene Symbol: BMP7
• Gene ID (NCBI): 655
• RRID: AB_2063960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P18075
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.

BMP7, also known as osteogenic protein-1 or OP-1, plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP7 may be involved in bone homeostasis (PMID: 15621726). It is expressed in the brain, kidneys and bladder. BMP7 is also present in cancers, including breast, prostate, and colon cancers, in which it is implicated in regulating cancer cell proliferation (PMID: 16419056, PMID: 15531927). Overexpression of BMP7 mRNA in colorectal cancer patients was significantly associated with poor prognosis and low overall survival (PMID: 18259822). Recent studies suggest that high-expression level of BMP7 serves as a biomarker for poor prognosis for HCC (PMID: 23179403).

Publications

Species	Application	Title
	WB	Circ Res; Yin Yang 1 Suppresses Dilated Cardiomyopathy and Cardiac Fibrosis Through Regulation of Bmp7 and Ctgf.; Authors - Chia Yee Tan
	WB,IF	Cell Mol Life Sci; Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7; Authors - Huijun Yang
rat	WB	Nanoscale; Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model.; Authors - Siwen Zhang
	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
rat	WB,IHC,IF	Front Pharmacol; BMP-7 Upregulates Id2 Through the MAPK Signaling Pathway to Improve Diabetic Tubulointerstitial Fibrosis and the Intervention of Oxymatrine.; Authors - Yawen Xiao
	WB	BMC Complement Med Ther; Huangqi decoction ameliorates kidney injury in db/db mice by regulating the BMP/Smad signaling pathway; Authors - Ying Chen