BRCA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BRCA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Publications

33

Reviews (1)

Cat no : 22362-1-AP

Synonyms

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 22362-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22362-1-AP (BRCA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 22362-1-AP

  IP result of anti-BRCA1 (IP:22362-1-AP, 4ug; Detection:22362-1-AP 1:6000) with MCF-7 cells lysate 760 ug.

• 

  IHC staining of rat testis using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22362-1-AP (BRCA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22362-1-AP (BRCA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22362-1-AP (BRCA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22362-1-AP (BRCA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22362-1-AP (BRCA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22362-1-AP (BRCA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22362-1-AP (BRCA1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22362-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22362-1-AP (BRCA1 Antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, rat testis tissue, mouse testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

22362-1-AP targets BRCA1 in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRCA1 fusion protein Ag17946
• Full Name: breast cancer 1, early onset
• Calculated Molecular Weight: 1863 aa, 208 kDa
• Observed Molecular Weight: 300 kDa
• GenBank Accession Number: BC115037
• Gene Symbol: BRCA1
• Gene ID (NCBI): 672
• RRID: AB_2879090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Publications

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao ; KD Validated
human	WB	Nat Commun; SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.; Authors - Lei Li ; KD Validated
human	IF	Nucleic Acids Res; USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.; Authors - Xia Ting
		Oxid Med Cell Longev; Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence.; Authors - Yujie Zhang
human	WB	Elife; Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.; Authors - Kerrie-Ann McMahon
human	WB,IHC	Oncogene; PTEN deficiency sensitizes endometrioid endometrial cancer to compound PARP-PI3K inhibition but not PARP inhibition as monotherapy.; Authors - X Bian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hasan (Verified Customer) (08-08-2022)

Western blot for HeLa cells transfected with siRNA against BRCA1 or control. anti-BRCA1 Ab (22362-1-AP) was used at dilution of (1:2000) in 5% skimmed milk. incubation overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa cells

BRCA1 Antibody Western Blot validation (1:2000 dilution) in HeLa cells (Cat no:22362-1-AP)