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BRCA1 Polyclonal antibody

BRCA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22362-1-AP

Synonyms

BRCA1, BRCAI, BRCC1, breast cancer 1, early onset, BROVCA1, IRIS, PSCP, RING finger protein 53, RNF53



Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22362-1-AP is BRCA1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRCA1 fusion protein Ag17946
Full Name breast cancer 1, early onset
Calculated molecular weight 1863 aa, 208 kDa
Observed molecular weight 300 kDa
GenBank accession numberBC115037
Gene symbol BRCA1
Gene ID (NCBI) 672
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRCA1, also named as RNF53, plays a central role in DNA repair by facilitating cellular response to DNA repair. It is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. It is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is required for FANCD2 targeting to sites of DNA damage. It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. The antibody is specific to BRCA1. BRCA1 appears to produce multiple splice variants. BRCA1 is a nuclear protein with a molecular mass of 220 kDa. The present study describes the isolation and expression of two cDNAs of BRCA1, including a splice variant designated BRCA1D672-4095. BRCA1D672-4095 is generated by exclusion of exon 11 by in-frame splicing and produces a 97 kDa protein. In contrast to BRCA1, BRCA1D672-4095 localizes to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for BRCA1 antibody 22362-1-APDownload protocol
IHC protocol for BRCA1 antibody 22362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oxid Med Cell Longev

Slowly Repaired Bulky DNA Damages Modulate Cellular Redox Environment Leading to Premature Senescence.

Authors - Yujie Zhang
humanIHC

Oncol Lett

Association of HMGB1, BRCA1 and P62 expression in ovarian cancer and chemotherapy sensitivity.

Authors - Shouyong Li
humanWB

Life Sci

BRCA1 protects cardiac microvascular endothelial cells against irradiation by regulating p21-mediated cell cycle arrest.

Authors - Zhi-Min Zeng
humanWB,IHC

Oncogene

PTEN deficiency sensitizes endometrioid endometrial cancer to compound PARP-PI3K inhibition but not PARP inhibition as monotherapy.

Authors - X Bian
humanIF

Nucleic Acids Res

USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.

Authors - Xia Ting
humanWB

Nat Commun

SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.

Authors - Lei Li
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