BRCC3 Recombinant antibody

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  WB analysis using 85430-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85430-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85430-4-RR (BRCC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

BRCC3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85430-4-RR

Clone No.242931B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BRCA1/BRCA2-containing complex subunit 3, BRCA1/BRCA2-containing complex subunit 36, BRCA1-A complex subunit BRCC36, BRCC36, BRISC complex subunit BRCC36

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Tested Applications

• Positive WB detected in: SK-BR-3 cells, HeLa cells, HEK-293T cells, MCF-7 cells, HT-29 cells, Neuro-2a cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85430-4-RR targets BRCC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BRCC3 fusion protein Ag7584
• Full Name: BRCA1/BRCA2-containing complex, subunit 3
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002999
• Gene Symbol: BRCC3
• Gene ID (NCBI): 79184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46736
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)