BRD1/BRL Polyclonal antibody

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  WB analysis using 32452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32452-1-AP (BRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 32452-1-AP

  IP result of anti-BRD1/BRL (IP:32452-1-AP, 4ug; Detection:32452-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

BRD1/BRL Polyclonal Antibody for WB, IP, ELISA

Cat No. 32452-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

BR140-like protein, BRD1, BRL, Bromodomain and PHD finger-containing protein 2, Bromodomain-containing protein 1

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Tested Applications

• Positive WB detected in: Jurkat cells, SH-SY5Y cells, SK-N-SH cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32452-1-AP targets BRD1/BRL in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37530

  Product name: Recombinant human BRD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 918-1070 aa of BC047508

  Sequence: VLPRLETLLQPRKRSRSTCGDSEVEEESPGKRLDAGLTNGFGGARSEQEPGGGLGRKATPRRRCASESSISSSNSPLCDSSFNAPKCGRGKPALVRRHTLEDRSELISCIENGNYAKAARIAAEVGQSSMWISTDAAASVLEPLKVVWAKCSG

• Full Name: bromodomain containing 1
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 133 kDa
• GenBank Accession Number: BC047508
• Gene Symbol: BRD1
• Gene ID (NCBI): 23774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95696
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD1, also known as BRL and BRPF2, plays a key role in chromatin recruitment to modulate histone acetylation and consequently gene transcription. It interacts with a range of epigenetic modifiers, including histone acetyltransferases KAT5 and KAT7, as well as the histone-lysine N-methyltransferase KMT5B (PMID: 29453316, 38383216). It has 2 isoforms with the molecular mass of 133 kDa and 120 kDa.