BRD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

BRD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 11859-1-AP

Synonyms

RING3-like protein, RING3L, RING3 like protein, ORFX, KIAA0043

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Validation Data Gallery

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  WB analysis using 11859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11859-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11859-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11859-1-AP

  IP result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug.

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11859-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11859-1-AP (BRD3 antibody) at dilution of 1:25 (under 10x lens).

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  IF Staining of HeLa using 11859-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, COLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 2 publications below

Product Information

11859-1-AP targets BRD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRD3 fusion protein Ag2433
• Full Name: bromodomain containing 3
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC032124
• Gene Symbol: BRD3
• Gene ID (NCBI): 8019
• RRID: AB_2065902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).

Publications

Species	Application	Title
human	WB	Nat Med; Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.; Authors - Xiangpeng Dai
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB	Sci Adv; Light-induced control of protein destruction by opto-PROTAC.; Authors - Jing Liu
human	WB	J Am Chem Soc; Cancer Selective Target Degradation by Folate-Caged PROTACs.; Authors - Jing Liu
human	WB	Nat Commun; Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer.; Authors - Wenyu Wang
human	WB	Nat Commun; A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.; Authors - Satoshi Yamanaka

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (02-06-2019)

Works well on fractionation lysates of MCF-7 cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7

BRD3 Antibody Western Blot, validation (1:1000 dilution) in MCF-7 (Cat no:11859-1-AP)