Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, COLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11859-1-AP targets BRD3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2433 Product name: Recombinant human BRD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC032124 Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM Predict reactive species |
| Full Name | bromodomain containing 3 |
| Calculated Molecular Weight | 556 aa, 61 kDa |
| Observed Molecular Weight | 95-105 kDa |
| GenBank Accession Number | BC032124 |
| Gene Symbol | BRD3 |
| Gene ID (NCBI) | 8019 |
| RRID | AB_2065902 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BRD3 antibody 11859-1-AP | Download protocol |
| IHC protocol for BRD3 antibody 11859-1-AP | Download protocol |
| IP protocol for BRD3 antibody 11859-1-AP | Download protocol |
| WB protocol for BRD3 antibody 11859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
11859-1-AP is a BRD4 antibody with 36 total citations published in journals including Nature Medicine, Nature Communications, Science Advances, Oncogene, Journal of the American Chemical Society, Molecular Cell, Acta Pharmaceutica Sinica B, and Haematologica. Associated research fields cover cancer biology (leukemia, neuroblastoma, colorectal, breast, prostate, and lung cancers), BET inhibitor and PROTAC degrader development, epigenetic regulation, chromatin remodeling, and targeted protein degradation strategies.
| Species | Application | Title |
|---|---|---|
Nat Med Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. | ||
Nat Commun The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis.
| ||
Nat Commun A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues. | ||
Nat Commun Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer. | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user reported using it at a 1:1000 dilution on MCF-7 cell fractionation lysates for Western blotting and found it worked well. Overall, the feedback indicates reliable performance for WB applications in cell lysates.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zehua (Verified Customer) (02-06-2019) | Works well on fractionation lysates of MCF-7 cells.
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