BTBD16 Polyclonal antibody

BTBD16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24733-1-AP

Synonyms

BTB (POZ) domain containing 16, BTBD16, C10orf87

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Validation Data Gallery

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  WB analysis of mouse testis using 24733-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24733-1-AP (BTBD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24733-1-AP (BTBD16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24733-1-AP (BTBD16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24733-1-AP is BTBD16 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTBD16 fusion protein Ag20672
• Full Name: BTB (POZ) domain containing 16
• Calculated Molecular Weight: 507 aa, 59 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC109262
• Gene Symbol: BTBD16
• Gene ID (NCBI): 118663
• RRID: AB_2879696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTBD16 also named as BTB (POZ) domain containing 16 or C10orf87 is 506 amino acid protein, which one BTB domain. The molecular weight fo BTBD16 is 58kDa. The function of BTBD16 protein is still non-known.