C10orf68 Polyclonal antibody

C10orf68 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24975-1-AP

Synonyms

C10orf68

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Validation Data Gallery

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  WB analysis of A549 using 24975-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 24975-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 24975-1-AP (C10orf68 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human kidney using 24975-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24975-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24975-1-AP (C10orf68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24975-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HEK-293 using 24975-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24975-1-AP (C10orf68 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, COS-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24975-1-AP is C10orf68 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C10orf68 fusion protein Ag21682
• Full Name: chromosome 10 open reading frame 68
• Calculated Molecular Weight: 628 aa, 71 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC125091
• Gene Symbol: C10orf68
• Gene ID (NCBI): 79741
• RRID: AB_2879828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

chromosome 10 open reading frame 68 (C10orf68) is a 628 amino-acid protein, which may have three isoforms. The function of C10orf68 is still non-known and needs to be further studied.