C10orf76 Polyclonal antibody

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  WB analysis using 33627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33627-1-AP (C10orf76 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

C10orf76 Polyclonal Antibody for WB, ELISA

Cat No. 33627-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Armadillo-like helical domain-containing protein 3, ARMH3

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33627-1-AP targets C10orf76 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38164

  Product name: Recombinant human C10orf76 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 539-689 aa of NM_024541.2

  Sequence: DTFLPTPSSYDELYYEIIRMHQSFDNLYSMVLRLSTNAGQWKEAASKVTHALVNIRAIINHFNPKIESYAAVNHISQLSEEQVLEVVRANYDTLTLKLQDGLDQYERYSEQHKEAAFFKELVRSISTNVRRNLAFHTLSQEVLLKEFSTIS

• Full Name: chromosome 10 open reading frame 76
• Calculated Molecular Weight: 79kDa，689aa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: NM_024541.2
• Gene Symbol: C10orf76
• Gene ID (NCBI): 79591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5T2E6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C10orf76, also known as ARMH3 (Armadillo-like helical domain-containing protein 3), was previously reported to be a regulator of phosphatidylinositol 4-kinase beta (PI4KB, also known as PI4KIIIbeta) at the TGN. Upon cGAMP-triggered translocation, ARMH3 interacted with STING at the Golgi and recruited phosphatidylinositol 4-kinase beta (PI4KB) to synthesize PI4P, which directed STING Golgi-to-endosome trafficking via PI4P-binding proteins AP-1 and GGA2 (PMID: 39580461, 36921576).