HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 84708-1-RR
Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).
HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 84708-1-RR
Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
C12orf29 Recombinant antibody
Catalog Number
84708-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
human, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
