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C13orf30 Polyclonal antibody

C13orf30 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25583-1-AP

Synonyms

Protein FAM216B, FAM216B

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using C13orf30 Polyclonal antibody (25583-1-AP)

    IHC staining of human kidney using 25583-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25583-1-AP (C13orf30 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using C13orf30 Polyclonal antibody (25583-1-AP)

    IHC staining of human kidney using 25583-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25583-1-AP (C13orf30 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using C13orf30 Polyclonal antibody (25583-1-AP)

    IF Staining of MDCK using 25583-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using C13orf30 antibody (25583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25583-1-AP targets C13orf30 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C13orf30 fusion protein Ag22353
Full Name chromosome 13 open reading frame 30
Calculated Molecular Weight 139 aa, 16 kDa
GenBank Accession NumberBC093659
Gene Symbol C13orf30
Gene ID (NCBI) 144809
RRIDAB_2880139
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for C13orf30 antibody 25583-1-APDownload protocol
IF protocol for C13orf30 antibody 25583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols