C17orf46 Polyclonal antibody

C17orf46 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24629-1-AP

Synonyms

C17orf46

---

Validation Data Gallery

• 

  WB analysis of L02 using 24629-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 24629-1-AP (C17orf46 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24629-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24629-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 24629-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24629-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24629-1-AP (C17orf46 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human testis tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24629-1-AP is C17orf46 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C17orf46 fusion protein Ag18239
• Full Name: chromosome 17 open reading frame 46
• Calculated Molecular Weight: 384 aa, 42 kDa
• Observed Molecular Weight: 42-52 kDa
• GenBank Accession Number: BC028124
• Gene Symbol: C17orf46
• Gene ID (NCBI): 124783
• RRID: AB_2879646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C17orf46, also named as SPATA32 and TEX34, mainly expressed in Testis. It interacts with syntaxin-1 and ACTB. It's abou 42-52 kDa in our detection.