C1qA Polyclonal antibody

C1qA Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11602-1-AP

Synonyms

C1qA

---

Validation Data Gallery

• 

  WB analysis of human spleen using 11602-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11602-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11602-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 11602-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse serum using 11602-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 11602-1-AP (C1QA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11602-1-AP

  IP Result of anti-C1QA (IP:11602-1-AP, 4ug; Detection:11602-1-AP 1:300) with mouse liver tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: human spleen tissue, human colon tissue, human heart tissue, human liver tissue, human lung tissue, mouse liver tissue, mouse serum tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below

Product Information

11602-1-AP targets C1qA in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1qA fusion protein Ag2141
• Full Name: complement component 1, q subcomponent, A chain
• Calculated molecular weight: 245 aa, 26 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC030153
• Gene symbol: C1QA
• Gene ID (NCBI): 712
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
mouse	WB	Cell Mol Life Sci; Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in mouse models of Parkinson's disease; Authors - Wenlong Zhang
mouse	WB	J Neurosci; Immune quiescence of the brain is set by astroglial connexin 43.; Authors - Anne-Cécile Boulay
rat	IF,WB	Neurochem Res; Blocking Two-Pore Domain Potassium Channel TREK-1 Inhibits the Activation of A1-Like Reactive Astrocyte Through the NF-κB Signaling Pathway in a Rat Model of Major Depressive Disorder; Authors - Ting Cong
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	Oncol Lett; Role of uromodulin and complement activation in the progression of kidney disease.; Authors - Fei Shen

Related Products

C1qC Monoclonal antibody

66268-1-Ig

C1qC Polyclonal antibody

16889-1-AP

C1R Polyclonal antibody

17346-1-AP

C1S Polyclonal antibody

14554-1-AP