C1qC Polyclonal antibody

C1qC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16889-1-AP

Synonyms

C1Q C, C1qC, C1QG



Tested Applications

Positive WB detected inhuman blood tissue, human blood, mouse serum
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 16889-1-AP is C1qC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1qC fusion protein Ag10400
Full Name complement component 1, q subcomponent, C chain
Calculated molecular weight 245 aa, 26 kDa
Observed molecular weight 24-26 kDa
GenBank accession numberBC009016
Gene symbol C1QC
Gene ID (NCBI) 714
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Protocols

Product Specific Protocols
WB protocol for C1qC antibody 16889-1-APDownload protocol
IHC protocol for C1qC antibody 16889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Surg Oncol

Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma

Authors - Wei Dong