RALGAPA2 Polyclonal antibody

RALGAPA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29843-1-AP

Synonyms

250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2

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Validation Data Gallery

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  WB analysis using 29843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 29843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HUVEC cells, mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29843-1-AP targets RALGAPA2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RALGAPA2 fusion protein Ag31460
• Full Name: chromosome 20 open reading frame 74
• Calculated Molecular Weight: 211KD
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: NM_020343
• Gene Symbol: C20orf74
• Gene ID (NCBI): 57186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.