C21orf66 Polyclonal antibody, PBS Only

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  WB analysis of human brain using 21357-1-AP (same clone as 21357-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 21357-1-AP (same clone as 21357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 21357-1-AP (same clone as 21357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 21357-1-AP (same clone as 21357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 21357-1-AP (same clone as 21357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21357-1-AP (same clone as 21357-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C21orf66 antibody (21357-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

C21orf66 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21357-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PAX3- and PAX7-binding protein 1, GC-rich sequence DNA-binding factor 1, GCFC1, GCFC, fSAP105

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Product Information

21357-1-PBS targets C21orf66 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C21orf66 fusion protein Ag15789
• Full Name: chromosome 21 open reading frame 66
• Calculated Molecular Weight: 917 aa, 105 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030539
• Gene Symbol: C21orf66
• Gene ID (NCBI): 94104
• RRID: AB_10733239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5B6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.