C21orf66 Polyclonal antibody

C21orf66 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21357-1-AP

Synonyms

PAX3- and PAX7-binding protein 1, GC-rich sequence DNA-binding factor 1, GCFC1, GCFC, fSAP105

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Validation Data Gallery

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  WB analysis of human brain using 21357-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 21357-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 21357-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 21357-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 21357-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21357-1-AP

  Immunofluorescent analysis of Hela cells, using C21orf66 antibody 21357-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human renal cell carcinoma tissue, human pancreas cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

21357-1-AP targets C21orf66 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C21orf66 fusion protein Ag15789
• Full Name: chromosome 21 open reading frame 66
• Calculated Molecular Weight: 917 aa, 105 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: BC030539
• Gene Symbol: C21orf66
• Gene ID (NCBI): 94104
• RRID: AB_10733239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes; Authors - Wenting Li
mouse	IHC	J Invest Dermatol; Paxbp1 is indispensable for the maintenance of epidermal homeostasis; Authors - Cong Huang
mouse	WB	Immunology; Paxbp1 is indispensable for the maintenance of peripheral CD4 T cell homeostasis; Authors - Wenting Li