Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human pancreas cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21357-1-AP targets C21orf66 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15789 Product name: Recombinant human C21orf66 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC030539 Sequence: MFRKARRVNVRKRNDSEEEERERDEEQEPPPLLPPPGTGEEAGPGGGDRAPGGESLLGPGPSPPSALTPGLGAEAGGGFPGGAEPGNGLKPRKRPRENKEVPRASLLSFQDEEEENEEVFKVKKSSYSKKIVKLLKKEYKEDLEKSKIKTELNSSAESEQPLDKTGHVKDTNQEDGVIISEHGEDEMDMESEKEEEKPKTGGAFSNALSSLNVLRPGEIPDAAFIHAARKKRQMARELGDFTPHDNEPGKGRLVREDENDASDDEDDDEKRRIVFSVKEKSQRQKIAEEIGIEGSDDDALVTGEQDEELSRWEQEQIRKGINIPQVQASQPAEVNMYYQNTYQTMPYGSS Predict reactive species |
| Full Name | chromosome 21 open reading frame 66 |
| Calculated Molecular Weight | 917 aa, 105 kDa |
| Observed Molecular Weight | 93 kDa |
| GenBank Accession Number | BC030539 |
| Gene Symbol | C21orf66 |
| Gene ID (NCBI) | 94104 |
| RRID | AB_10733239 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5B6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C21orf66 antibody 21357-1-AP | Download protocol |
| IHC protocol for C21orf66 antibody 21357-1-AP | Download protocol |
| IP protocol for C21orf66 antibody 21357-1-AP | Download protocol |
| WB protocol for C21orf66 antibody 21357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Paxbp1 antibody (Cat# 21357-1-AP) has 3 citations, appearing in the Journal of Investigative Dermatology, Frontiers in Immunology, and Immunology. All cited studies use mouse models with IHC or WB applications. The research fields span epidermal homeostasis, thymocyte survival, and peripheral CD4 T cell homeostasis, highlighting the antibody's utility in dermatology and T cell immunology.
| Species | Application | Title |
|---|---|---|
Front Immunol Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes | ||
Immunology Paxbp1 is indispensable for the maintenance of peripheral CD4 T cell homeostasis |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot on liver tissue at a 1:1000 dilution and reported that it "looks good for Western," indicating satisfactory performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-07-2025) | LOOKS GOOD FOR WESTERN
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