Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse brain tissue, Jurkat cells, K-562 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12391-1-AP targets CA8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CA8 fusion protein Ag3068|
|Full Name||carbonic anhydrase VIII|
|Calculated molecular weight||290 aa, 32 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC015531|
|Gene ID (NCBI)||767|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).
Nucleic Acids Res
Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.
J Am Soc Nephrol
Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.
CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.
Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.
Am J Physiol Gastrointest Liver Physiol
Carbonic anhydrase 8 (CAR8) negatively regulates GLP-1 secretion from enteroendocrine cells in response to long-chain fatty acids.
Disruption of the ATXN1-CIC complex reveals the role of additional nuclear ATXN1 interactors in spinocerebellar ataxia type 1