CA8 Polyclonal antibody

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CA8 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12391-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

carbonic anhydrase VIII, CA 8, CA VIII, CALS, Carbonic anhydrase-related protein

• 

  WB analysis using 12391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 12391-1-AP

  WB result of CA8 antibody (12391-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA8 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 12391-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12391-1-AP

  IP result of anti-CA8 (IP:12391-1-AP, 4ug; Detection:12391-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, Jurkat cells, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12391-1-AP targets CA8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3068

  Product name: Recombinant human CA8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-143 aa of BC015531

  Sequence: MADLSFIEDTVAFPEKEEDEEEEEEGVEWGYEEGVEWGLVFPDANGEYQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLSGGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPMEVRIT

• Full Name: carbonic anhydrase VIII
• Calculated Molecular Weight: 290 aa, 32 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC015531
• Gene Symbol: CA8
• Gene ID (NCBI): 767
• RRID: AB_2066277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35219
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

Publications

What published studies show

The CA8 Polyclonal antibody (Cat# 12391-1-AP) has 11 total citations. These appear in journals including Cell Research, Nature Communications, Neuron, Nucleic Acids Research, Journal of the American Society of Nephrology, Molecular Oncology, Biomedical Pharmacotherapy, Cell Biochemistry and Function, American Journal of Physiology, and PLoS One. Research fields span neuroscience (cerebellar function, spinocerebellar ataxia, brain development), oncology (colorectal and renal cell carcinoma), renal physiology, RNA-mediated gene silencing, and metabolic regulation.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Cell Res; Cochlear nucleus spatial transcriptomes of normal and hearing loss mice reveal a critical role of Spp1 in bushy cells.; Authors - Huihui Liu
mouse	IF	Nat Commun; Glutamatergic cerebellar neurons differentially contribute to the acquisition of motor and social behaviors; Authors - Meike E van der Heijden
mouse	IHC	Neuron; Disruption of the ATXN1-CIC complex reveals the role of additional nuclear ATXN1 interactors in spinocerebellar ataxia type 1; Authors - Stephanie L Coffin
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada ; KD Validated
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	IHC	Mol Oncol; Consensus molecular subtyping of colorectal carcinoma brain metastases reveals a metabolic signature associated with poor patient survival; Authors - Barnabas Irmer