CA8 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA8 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12391-1-AP

Synonyms

CA VIII, CA8, CALS, carbonic anhydrase VIII, CARP

---

Validation Data Gallery

• 

  WB analysis using 12391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12391-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12391-1-AP (CA8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12391-1-AP

  IP result of anti-CA8 (IP:12391-1-AP, 4ug; Detection:12391-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12391-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12391-1-AP (CA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, Jurkat cells, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

12391-1-AP targets CA8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA8 fusion protein Ag3068
• Full Name: carbonic anhydrase VIII
• Calculated Molecular Weight: 290 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC015531
• Gene Symbol: CA8
• Gene ID (NCBI): 767
• RRID: AB_2066277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

Publications

Species	Application	Title
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada ; KD Validated
mouse	IF	J Am Soc Nephrol; Single-Cell RNA Sequencing Reveals Renal Endothelium Heterogeneity and Metabolic Adaptation to Water Deprivation.; Authors - Sébastien J Dumas
human	WB,IHC	Biomed Pharmacother; CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.; Authors - Huai-Lu Ma ; KD Validated
rat	IHC	PLoS One; Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.; Authors - Liddelow Shane A SA
mouse	IHC	Am J Physiol Gastrointest Liver Physiol; Carbonic anhydrase 8 (CAR8) negatively regulates GLP-1 secretion from enteroendocrine cells in response to long-chain fatty acids.; Authors - Yuta Fujiwara
mouse	IHC	Neuron; Disruption of the ATXN1-CIC complex reveals the role of additional nuclear ATXN1 interactors in spinocerebellar ataxia type 1; Authors - Stephanie L Coffin