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CACNA1A Polyclonal antibody

CACNA1A Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 27227-1-AP

Synonyms

APCA, BI, Brain calcium channel I, CACH4, CACN3, CACNA1A, CACNL1A4, CAV2.1, EA2, FHM, HPCA, MHP, MHP1, SCA6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1A Polyclonal antibody (27227-1-AP)

    IHC staining of human brain using 27227-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27227-1-AP (CACNA1A Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using CACNA1A Polyclonal antibody (27227-1-AP)

    IHC staining of human brain using 27227-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27227-1-AP (CACNA1A Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27227-1-AP is CACNA1A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA1A fusion protein Ag25943
Full Name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Calculated Molecular Weight 282 kDa
GenBank Accession NumberNM_000068
Gene Symbol CACNA1A
Gene ID (NCBI) 773
RRIDAB_2880810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CACNA1A antibody 27227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ümit (Verified Customer) (08-11-2022)

Cav2.1 (black) staining of two rhesus monkey brainstem sections (5µm &7µm) visualized with immunoperoxidase method (DAB-Nickel).

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Monkey/Purkinje Cells
CACNA1A Antibody Immunohistochemistry validation (1:100 dilution) in Monkey/Purkinje Cells (Cat no:27227-1-AP)