CaMKIV Polyclonal antibody

CaMKIV Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13263-1-AP

Synonyms

CaM kinase GR, CaMK GR, CAMK4, CaMKIV

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Validation Data Gallery

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  WB analysis of human cerebellum using 13263-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13263-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13263-1-AP (CaMKIV antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13263-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13263-1-AP (CaMKIV antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human cerebellum tissue, mouse thymus tissue
• Positive IHC detected in: human ovary tumor tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

13263-1-AP targets CaMKIV in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CaMKIV fusion protein Ag3922
• Full Name: calcium/calmodulin-dependent protein kinase IV
• Calculated Molecular Weight: 473 aa, 52 kDa
• Observed Molecular Weight: 56-65 kDa
• GenBank Accession Number: BC025687
• Gene Symbol: CAMK4
• Gene ID (NCBI): 814
• RRID: AB_2068381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)

Publications

Species	Application	Title
human	WB	Oncotarget; PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.; Authors - Chao Li
human	WB	J Invest Dermatol; The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes.; Authors - Shiyao Pei
mouse	WB	Toxicol Res (Camb); Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers.; Authors - Disi Bai
rat	WB,IF	Cell Death Discov; Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.; Authors - Qingying Tang