CAMLG Polyclonal antibody

CAMLG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 23327-1-AP

Synonyms

calcium modulating ligand, CAML, CAMLG

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Validation Data Gallery

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  WB analysis of Jurkat using 23327-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 23327-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 23327-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23327-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23327-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23327-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23327-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23327-1-AP is CAMLG Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMLG fusion protein Ag19915
• Full Name: calcium modulating ligand
• Calculated Molecular Weight: 296 aa, 33 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC130325
• Gene Symbol: CAMLG
• Gene ID (NCBI): 819
• RRID: AB_2879256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (06-28-2020)

Sharp band at predicted size.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart