CAMP Polyclonal antibody

CAMP Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12009-1-AP

Synonyms

CAMP, CAP 18, CAP18, CRAMP, FALL 39, FALL39, hCAP 18, HSD26, LL37



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12009-1-AP targets CAMP in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMP fusion protein Ag2622
Full Name cathelicidin antimicrobial peptide
Calculated molecular weight 170 aa, 19 kDa
GenBank accession numberBC055089
Gene symbol CAMP
Gene ID (NCBI) 820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

Protocols

Product Specific Protocols
IHC protocol for CAMP antibody 12009-1-APDownload protocol
IF protocol for CAMP antibody 12009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Res Vet Sci

Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.

Authors - Lingxia Li
mouseIHC

Immunobiology

IL-33 synergistically promotes the proliferation of lung cancer cells in vitro by inducing antibacterial peptide LL-37 and proinflammatory cytokines in macrophages.

Authors - Yinting Jiang
  • KD Validated
mouseIF

EMBO Mol Med

Gut microbiota-CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten-induced enteropathy.

Authors - Zhengnan Ren
  • KO Validated