CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

6B10F11

Cat no : 66352-1-Ig

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66352-1-Ig targets CAPRIN1 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7413
Full Name cell cycle associated protein 1
Calculated molecular weight 78 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC001731
Gene symbol CAPRIN1
Gene ID (NCBI) 4076
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Protocols

Product Specific Protocols
WB protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
IHC protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
IF protocol for CAPRIN1 antibody 66352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Memg (Verified Customer) (01-10-2020)

using caprin1 antibody can't get signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:20000