CAPRIN1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66352-1-Ig (same clone as 66352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66352-1-Ig (CAPRIN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (66352-1-Ig, Clone: 6B10F11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66352-1-Ig (same clone as 66352-1-PBS)

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66352-1-Ig(CAPRIN1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 66352-1-Ig (same clone as 66352-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (66352-1-Ig, Clone:6B10F11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66352-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51429-1

  Cytometric bead array sample test of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS.

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  Cytometric bead array standard curve of MP51429-1

  Cytometric bead array standard curve of MP51429-1, CAPRIN1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66352-1-PBS. Detection antibody: 66352-2-PBS. Standard:Ag7413. Range: 1.563-200 ng/mL.

CAPRIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 66352-1-PBS

Clone No.6B10F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

6B10F11, caprin 1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1

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Product Information

66352-1-PBS targets CAPRIN1 as part of a matched antibody pair:

MP51429-1: 66352-1-PBS capture and 66352-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7413

  Product name: Recombinant human CAPRIN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 356-709 aa of BC001731

  Sequence: RQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN

• Full Name: cell cycle associated protein 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC001731
• Gene Symbol: CAPRIN1
• Gene ID (NCBI): 4076
• RRID: AB_2881732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14444
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.