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CoraLite® Plus 488-conjugated CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B10F11

Cat no : CL488-66352

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CAPRIN1 Monoclonal a (CL488-66352)

    IF Staining of HeLa using CL488-66352

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66352 (CAPRIN1 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 488-conjugated CAPRIN1 Monoclonal a (CL488-66352)

    FC experiment of NIH/3T3 using CL488-66352

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAPRIN1 (CL488-66352, Clone:6B10F11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66352 targets CAPRIN1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7413
Full Name cell cycle associated protein 1
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2883316
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CAPRIN1 antibody CL488-66352Download protocol
FC protocol for CL Plus 488 CAPRIN1 antibody CL488-66352Download protocol
Standard Protocols
Click here to view our Standard Protocols