CARD6 Polyclonal antibody

CARD6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18029-1-AP

Synonyms

CARD6, CINCIN1

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Validation Data Gallery

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  WB analysis of HepG2 using 18029-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18029-1-AP (CARD6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18029-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18029-1-AP (CARD6 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human testis tissue, human kidney tissue, human lung tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18029-1-AP targets CARD6 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CARD6 fusion protein Ag12595
• Full Name: caspase recruitment domain family, member 6
• Calculated Molecular Weight: 1037 aa, 116 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC093825
• Gene Symbol: CARD6
• Gene ID (NCBI): 84674
• RRID: AB_2290686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The caspase recruitment domain (CARD) is a homotypic protein-protein interaction module that links components of signal transduction pathways implicated in the regulation of apoptosis or adaptive or innate immunity. Although much progress has been made in assigning precise roles to most CARD-containing proteins, the functions of the 1,037-amino-acid (aa) human and 1,175-aa mouse CARD6 proteins are still unknown. CARD6 has a unique structure in that it contains the CARD at the N terminus, a glutamic acid-rich region following the CARD, and a proline-rich region at the C terminus. CARD6 also harbors a 350-aa region with similarity to upregulated gene 4 (URG4), a protein that is induced in response to hepatitis Bx antigen overexpression and exerts a positive effect on proliferation. Both CARD6 and URG4 share structural features with members of the multifaceted, IFN-inducible GTPase (IFNiGTPase) superfamily, which contains some of the proteins most abundantly induced during cell-autonomous immune responses. The calcualted molecular weight of CARD is 116 kDa, but modified CARD6 is about 130 kDa. (PMID: 18160713)

Publications

Species	Application	Title
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang