Caspase 3 Antibody 181 Publications

Rabbit Polyclonal| Catalog number: 19677-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 41 μg/150 μl

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Species specificity:
human, mouse, rat, Hamster, monkey, sheep

Positive WB detected in:
Jurkat cells, HeLa cells, mouse spleen tissue

Positive IP detected in:
NIH/3T3 cells

Positive IHC detected in:
mouse brain tissue, human kidney tissue, human spleen tissue, human teeth tissue

Positive IF detected in:
mouse brain tissue, HeLa cells, NIH/3T3 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
caspase 3, apoptosis-related cysteine peptidase

Calculated molecular weight:
32 kDa

Observed molecular weight:
32-35 kDa, 17 kDa, 19kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CASP3

Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Background

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17, p19 and p32 of Caspase 3. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa.(PMID 9747872)


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