Caspase 3/p17/p19 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1838

Reviews (6)

Cat no : 19677-1-AP

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase 3/p17/p19, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Validation Data Gallery

• 

  WB analysis using 19677-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19677-1-AP

  WB result of Caspase 3 antibody (19677-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells.

• 

  WB analysis of mouse spleen using 19677-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis using 19677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 19677-1-AP

  IP result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

• 

  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 19677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 19677-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Caspase 3/p17/p19 (19677-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells, mouse brain tissue, HeLa cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 1697 publications below
• IHC: See 167 publications below
• IF: See 82 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat, chicken, sheep, monkey, rabbit, Zebrafish, Hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 32-35 kDa, 17 kDa, 19 kDa
• GenBank accession number: NM_004346
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
	WB	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
mouse	WB	Theranostics; Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation; Authors - Zongqi You

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC34

FH

Hala (Verified Customer) (04-12-2021)

works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepg2 treated with ethoposide

FH

Isha (Verified Customer) (02-03-2021)

Gave me crystal clear bands in kidney lysates. really happy with the product

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney

FH

Diane (Verified Customer) (02-02-2021)

Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cell lysates - glioblastoma primary

Caspase 3/p17/p19 Antibody Western Blot validation (1:500 dilution) in Cell lysates - glioblastoma primary (Cat no:19677-1-AP)

FH

Chao (Verified Customer) (03-12-2020)

Full-length but not cleaved isoform is detected by western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Kishor (Verified Customer) (01-30-2019)

It is an excellent antibody, worked every time when I used and got satisfactory results.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Caspase 3/p17/p19 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:19677-1-AP)

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