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Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, RIP, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
1838
Cat no : 19677-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, mouse brain tissue, HeLa cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 1697 publications below |
IHC | See 167 publications below |
IF | See 82 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
RIP | See 1 publications below |
Product Information
19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, chicken, sheep, monkey, rabbit, Zebrafish, Hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | caspase 3, apoptosis-related cysteine peptidase |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 32-35 kDa, 17 kDa, 19 kDa |
GenBank accession number | NM_004346 |
Gene symbol | CASP3 |
Gene ID (NCBI) | 836 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
Neuron UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. | ||
Bone Res Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma | ||
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI | ||
Theranostics Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.
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FH Hala (Verified Customer) (04-12-2021) | works very well
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FH Isha (Verified Customer) (02-03-2021) | Gave me crystal clear bands in kidney lysates. really happy with the product
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FH Diane (Verified Customer) (02-02-2021) | Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.
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FH Chao (Verified Customer) (03-12-2020) | Full-length but not cleaved isoform is detected by western blot
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FH Kishor (Verified Customer) (01-30-2019) | It is an excellent antibody, worked every time when I used and got satisfactory results.
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