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  • KD/KO Validated

Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19677-1-AP

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1



Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, mouse brain tissue, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Bovine, canine, chicken, duck, Goat, hamster, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 32 kDa
Observed molecular weight 32-35 kDa, 17 kDa, 19 kDa
GenBank accession numberNM_004346
Gene symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Protocols

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IP protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
FC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
WB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
humanWB

Nat Commun

Structural insight into the molecular mechanism of p53-mediated mitochondrial apoptosis.

Authors - Hudie Wei
humanWB

Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
humanWB

Nat Commun

Structural and functional analyses of hepatitis B virus X protein BH3-like domain and Bcl-xL interaction.

Authors - Tian-Ying Zhang
humanWB

Autophagy

Inhibition of autophagy enhances the anticancer activity of silver nanoparticles.

Authors - Jun Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSC34
FH

Hala (Verified Customer) (04-12-2021)

works very well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepg2 treated with ethoposide
FH

Isha (Verified Customer) (02-03-2021)

Gave me crystal clear bands in kidney lysates. really happy with the product

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Kidney
FH

Diane (Verified Customer) (02-02-2021)

Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Cell lysates - glioblastoma primary
Caspase 3/p17/p19 Antibody Western Blot validation (1:500 dilution) in Cell lysates - glioblastoma primary (Cat no:19677-1-AP)
FH

Chao (Verified Customer) (03-12-2020)

Full-length but not cleaved isoform is detected by western blot

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Kishor (Verified Customer) (01-30-2019)

It is an excellent antibody, worked every time when I used and got satisfactory results.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
Caspase 3/p17/p19 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:19677-1-AP)