Caspase 8/p43/p18 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

225

Reviews (2)

Cat no : 13423-1-AP

Synonyms

Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MCH5

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Validation Data Gallery

• 

  WB analysis of Jurkat using 13423-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 13423-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13423-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13423-1-AP

  IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IP experiment of Jurkat using 13423-1-AP

  IP result of anti-Caspase 8 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13423-1-AP

  Immunofluorescent analysis of HepG2 cells using 13423-1-AP (Caspase 8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
• Positive IP detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human lymphoma tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 211 publications below
• IHC: See 14 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 8/p43/p18 fusion protein Ag4214
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated molecular weight: 538 aa, 62 kDa
• Observed molecular weight: 53-57 kDa, 32-45 kDa
• GenBank accession number: BC028223
• Gene symbol: CASP8
• Gene ID (NCBI): 841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Publications

Species	Application	Title
human	WB	J Cell Physiol; Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome.; Authors - Yulin Li
mouse	WB	Front Pharmacol; Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice.; Authors - Jingxin Mao
mouse	WB	Int Immunopharmacol; Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice.; Authors - Yutong He
human	WB	Int J Food Sci Nutr; Sulforaphane inhibits gastric cancer stem cells via suppressing sonic hedgehog pathway.; Authors - Miaomiao Ge
human	WB	J Biomed Nanotechnol; Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo.; Authors - Duoduo Zhang
human	WB,IHC	Sci Rep; Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.; Authors - Zhaoxing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: kidney

FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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