Caspase 8/p43/p18 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

244

Reviews (2)

Cat no : 13423-1-AP

Synonyms

Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MCH5

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Validation Data Gallery

• 

  WB analysis of Jurkat using 13423-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 13423-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13423-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13423-1-AP

  IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IP experiment of Jurkat using 13423-1-AP

  IP result of anti-Caspase 8 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13423-1-AP

  Immunofluorescent analysis of HepG2 cells using 13423-1-AP (Caspase 8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
• Positive IP detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human lymphoma tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 230 publications below
• IHC: See 17 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 8/p43/p18 fusion protein Ag4214
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated molecular weight: 538 aa, 62 kDa
• Observed molecular weight: 53-57 kDa, 32-45 kDa
• GenBank accession number: BC028223
• Gene symbol: CASP8
• Gene ID (NCBI): 841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Publications

Species	Application	Title
	IP	Sci Adv; HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.; Authors - Chun Gao
human	WB	Nat Commun; Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.; Authors - Christoph Grohmann
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang
human	WB	Autophagy; Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.; Authors - Xu Li
human	WB,IF	Mol Ther; Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.; Authors - Benfu Zhong
human	WB	Redox Biol; Yeast β-D-glucan exerts antitumour activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis.; Authors - Ningning Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: kidney

FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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