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Caspase 8/p43/p18 Polyclonal antibody
Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13423-1-AP
Validation Data Gallery
|Positive WB detected in
|Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
|Positive IP detected in
|HeLa cells, Jurkat cells
|Positive IHC detected in
|human lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, chicken, rabbit, pig
|Host / Isotype
|Rabbit / IgG
|Caspase 8/p43/p18 fusion protein Ag4214
|caspase 8, apoptosis-related cysteine peptidase
|Calculated molecular weight
|538 aa, 62 kDa
|Observed molecular weight
|53-57 kDa, 32-45 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
|Product Specific Protocols
|WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP
|IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP
|IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP
|IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xiaoping (Verified Customer) (12-15-2021)
Got really good bands in kidney lysates at 1:500 dilution
WEI (Verified Customer) (01-17-2020)
Good antibody for detecting IR injury-induced apoptosis in mouse heart.