Caspase 8/P43/P18 Polyclonal antibody

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  WB analysis of Jurkat using 13423-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 13423-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13423-1-AP

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IP experiment of Jurkat using 13423-1-AP

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.

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  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13423-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/P43/P18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Caspase 8/P43/P18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13423-1-AP

Publications(360)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, UV treated Hela, RAW264.7
• Positive IP detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human lymphoma tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 340 publications below
• IHC: See 20 publications below
• IF: See 12 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

13423-1-AP targets Caspase 8/P43/P18 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 8/P43/P18 fusion protein Ag4214
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• RRID: AB_2068463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
	IP	Sci Adv; HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.; Authors - Chun Gao
human	WB	Exp Mol Med; PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer; Authors - Gu-Choul Shin
human	WB	Nat Commun; Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.; Authors - Christoph Grohmann
human	WB	Aging Dis; MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.; Authors - Yanhui Cui
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: kidney

FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart