- Featured Product
- KD/KO Validated
Caspase 8/p43/p18 Polyclonal antibody
Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13423-1-AP
Validation Data Gallery
|Positive WB detected in||Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells|
|Positive IP detected in||HeLa cells, Jurkat cells|
|Positive IHC detected in||human lymphoma tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, chicken, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 8/p43/p18 fusion protein Ag4214|
|Full Name||caspase 8, apoptosis-related cysteine peptidase|
|Calculated molecular weight||538 aa, 62 kDa|
|Observed molecular weight||53-57 kDa, 32-45 kDa|
|GenBank accession number||BC028223|
|Gene ID (NCBI)||841|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.
|Product Specific Protocols|
|WB protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IHC protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IF protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|IP protocol for Caspase 8/p43/p18 antibody 13423-1-AP||Download protocol|
|Click here to view our Standard Protocols|
HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.
Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.
A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.
Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.
Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.
Yeast β-D-glucan exerts antitumour activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xiaoping (Verified Customer) (12-15-2021)
Got really good bands in kidney lysates at 1:500 dilution
WEI (Verified Customer) (01-17-2020)
Good antibody for detecting IR injury-induced apoptosis in mouse heart.