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Caspase 8/p43/p18 Polyclonal antibody

Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13423-1-AP

Synonyms

Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MCH5



Tested Applications

Positive WB detected inStaurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13423-1-AP targets Caspase 8/p43/p18 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 8/p43/p18 fusion protein Ag4214
Full Name caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight 538 aa, 62 kDa
Observed molecular weight 53-57 kDa, 32-45 kDa
GenBank accession numberBC028223
Gene symbol CASP8
Gene ID (NCBI) 841
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Protocols

Product Specific Protocols
WB protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IHC protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IF protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
IP protocol for Caspase 8/p43/p18 antibody 13423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Sci Adv

HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.

Authors - Chun Gao
humanWB

Nat Commun

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.

Authors - Christoph Grohmann
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang
humanWB

Autophagy

Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.

Authors - Xu Li
humanWB,IF

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong
humanWB

Redox Biol

Yeast β-D-glucan exerts antitumour activity in liver cancer through impairing autophagy and lysosomal function, promoting reactive oxygen species production and apoptosis.

Authors - Ningning Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: kidney
FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart