Name: Caspase 8 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

Caspase 8 Antibody WB Staurosporine treated Jurkat cells 13423-1-AP

Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Caspase 8 Antibody IP HeLa cells 13423-1-AP

IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

Caspase 8 Antibody IHC human lymphoma tissue 13423-1-AP

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP( Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP( Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 8 Antibody IF HepG2 cells 13423-1-AP

Immunofluorescent analysis of HepG2 cells using 13423-1-AP(Caspase 8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:

human, mouse, chicken, rat

Positive WB detected in:

Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, Jurkat cells, UV treated Hela, Sp2/0 cells

Positive IP detected in:

HeLa cells, Jurkat cells

Positive IHC detected in:

human lymphoma tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HepG2 cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:50-1:500

IF : 1:10-1:100

Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Caspase 8 fusion protein Ag4214

Full name:

caspase 8, apoptosis-related cysteine peptidase

Calculated molecular weight:

538aa,62 kDa

Observed molecular weight:

53-57 kDa, 32-45 kDa

GenBank accession number:

BC028223

Gene ID (NCBI):

841

Gene symbol

CASP8

Synonyms

Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MCH5

Background

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Showing 1- [6] of [94] Publications

Author:		Shi Yin
Pubmed ID:		27819521
Journal:		Cancer Biol Ther
Title:		MicroRNA-326 Sensitizes Human Glioblastoma Cells to Curcumin via the SHH/GLI1 Signaling Pathway.
Application:		WB
KD validated
Species:		human

Author:		Jianyun Zhu
Pubmed ID:		27836540
Journal:		Biochem Biophys Res Commun
Title:		Wnt/β-catenin pathway mediates (-)-Epigallocatechin-3-gallate (EGCG) inhibition of lung cancer stem cells.
Application:		WB

Species:		human

Author:		Gongsheng Yuan
Pubmed ID:		27883226
Journal:		J Bone Miner Res
Title:		The Circadian Gene Clock Regulates Bone Formation via PDIA3.
Application:		WB

Species:

Author:		Wei-Ye Shi
Pubmed ID:		27827850
Journal:		Int J Mol Sci
Title:		Interferon α Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced Pathway.
Application:		WB

Species:		human

Author:		Qin Tang
Pubmed ID:		27912880
Journal:		Phytomedicine
Title:		Combination of baicalein and 10-hydroxy camptothecin exerts remarkable synergetic anti-cancer effects.
Application:		WB

Species:		human

Author:		Yongyong Cui
Pubmed ID:		28018864
Journal:		Front Cell Infect Microbiol
Title:		Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in a Murine Macrophage Cell Line.
Application:		WB

Species:		mouse

WB Figures

Caspase 8 Antibody WB HeLa cells 13423-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP(CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB Jurkat cells 13423-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB Raji cells 13423-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP(CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB RAW264.7 13423-1-AP

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP(CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP(CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Caspase 8 Antibody WB UV treated Hela 13423-1-AP

UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP(CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

IHC Figures

Caspase 8 Antibody IHC human colon tissue 13423-1-AP

Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP(CASP8 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP(CASP8 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP(CASP8 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP(CASP8 antibody) at dilution of 1:50 (under 10x lens)

IP Figures

IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

Caspase 8 Antibody IP Jurkat cells 13423-1-AP

IP result of anti-Caspase 8(IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug

IF Figures

Immunofluorescent analysis of HepG2 cells using 13423-1-AP(Caspase 8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Caspase 8 Antibody 94 Publications

Rabbit Polyclonal| Catalog number: 13423-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [94] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

Caspase 8 Antibody 94 Publications

Rabbit Polyclonal| Catalog number: 13423-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background