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Caspase 8 Antibody 77 Publications

Rabbit Polyclonal| Catalog number: 13423-1-AP

Featured Product KD/KO validated

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Con: 70 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Caspase 8 Antibody WB Staurosporine treated Jurkat cells 13423-1-AP

Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Caspase 8 Antibody IP HeLa cells 13423-1-AP

IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

Caspase 8 Antibody IHC human lymphoma tissue 13423-1-AP

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 13423-1-AP( Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 8 Antibody IHC human lymphoma tissue 13423-1-AP

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 13423-1-AP( Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 8 Antibody IF HepG2 cells 13423-1-AP

Immunofluorescent analysis of HepG2 cells using 13423-1-AP(Caspase 8 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, chicken, rat
Positive WB detected in:
Staurosporine treated Jurkat cells, RAW264.7, HeLa cells, Raji cells, UV treated Hela
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lymphoma tissue, human colon tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caspase 8 fusion protein Ag4214
Full name:
caspase 8, apoptosis-related cysteine peptidase
Calculated molecular weight:
538aa,62 kDa
Observed molecular weight:
53-57 kDa, 32-45 kDa
GenBank accession number:
BC028223
Gene ID (NCBI):
841
Gene symbol
CASP8
Synonyms
Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, FADD like ICE, FLICE, ICE like apoptotic protease 5, MCH5

Background

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.


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