Validation Data Gallery
|Positive WB detected in||human heart tissue, human skeletal muscle tissue|
|Positive IHC detected in||human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cell|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18422-1-AP targets Calsequestrin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calsequestrin 2 fusion protein Ag13246|
|Full Name||calsequestrin 2 (cardiac muscle)|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC022288|
|Gene ID (NCBI)||845|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.
Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.
Mol Biol Cell
Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.
Stem Cell Reports
A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.
Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.
EMBO Mol Med
SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias.