Calsequestrin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Calsequestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 18422-1-AP

Synonyms

Calsequestrin 2, CASQ2, PDIB2

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Validation Data Gallery

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  WB analysis using 18422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18422-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 18422-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of C2C12 using 18422-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human skeletal muscle tissue, pig heart tissue, rat heart tissue
• Positive IHC detected in: human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cell

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

18422-1-AP targets Calsequestrin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calsequestrin 2 fusion protein Ag13246
• Full Name: calsequestrin 2 (cardiac muscle)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022288
• Gene Symbol: Calsequestrin 2
• Gene ID (NCBI): 845
• RRID: AB_2071473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias.; Authors - Shiraz Haron-Khun
mouse	WB,IF	JCI Insight; The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.; Authors - Daniel J Blackwell ; KO Validated
mouse	WB,IF	Heart Rhythm; Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.; Authors - Efrat Kurtzwald-Josefson ; KO Validated
human	WB	Stem Cell Reports; A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.; Authors - Matthew J Birket
		Mol Biol Cell; Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.; Authors - Josephine A Bonventre
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi