- Featured Product
- KD/KO Validated
Calsequestrin 2 Polyclonal antibody
Calsequestrin 2 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat, pig
WB, IHC, IF, ELISA
Cat no : 18422-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, human heart tissue, human skeletal muscle tissue, pig heart tissue, rat heart tissue|
|Positive IHC detected in||human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cell|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 5 publications below|
|IF||See 2 publications below|
18422-1-AP targets Calsequestrin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calsequestrin 2 fusion protein Ag13246|
|Full Name||calsequestrin 2 (cardiac muscle)|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC022288|
|Gene ID (NCBI)||845|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
|Product Specific Protocols|
|WB protocol for Calsequestrin 2 antibody 18422-1-AP||Download protocol|
|IHC protocol for Calsequestrin 2 antibody 18422-1-AP||Download protocol|
|IF protocol for Calsequestrin 2 antibody 18422-1-AP||Download protocol|
|Click here to view our Standard Protocols|
EMBO Mol Med
SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias.
The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.
Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.
Stem Cell Reports
A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.
Mol Biol Cell
Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.
Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.