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- KD/KO Validated
Calsequestrin 2 Polyclonal antibody
Calsequestrin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 18422-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, human heart tissue, human skeletal muscle tissue, pig heart tissue, rat heart tissue |
Positive IHC detected in | human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C2C12 cell |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 10 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
18422-1-AP targets Calsequestrin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Calsequestrin 2 fusion protein Ag13246 |
Full Name | calsequestrin 2 (cardiac muscle) |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC022288 |
Gene Symbol | Calsequestrin 2 |
Gene ID (NCBI) | 845 |
RRID | AB_2071473 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
Protocols
Product Specific Protocols | |
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WB protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
IHC protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
IF protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Mol Med SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias. | ||
JCI Insight The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.
| ||
Heart Rhythm Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.
| ||
Stem Cell Reports A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. | ||
Mol Biol Cell Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish. | ||
JCI Insight Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy. |