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Caveolin-1 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66067-1-Ig |CloneNo.: 6C2B2

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Con: 111 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Caveolin-1 Antibody WB A549 cells 66067-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours

Caveolin-1 Antibody WB HeLa cells 66067-1-Ig

WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells

Caveolin-1 Antibody IHC human skin cancer tissue 66067-1-Ig

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caveolin-1 Antibody IHC human skin cancer tissue 66067-1-Ig

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
A549 cells, HeLa cells
Positive IHC detected in:
human skin cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:50000
IHC : 1:2500-1:10000
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caveolin-1 fusion protein Ag8049
Full name:
caveolin 1, caveolae protein, 22kDa
Calculated molecular weight:
22 kDa
Observed molecular weight:
20-25 kDa
GenBank accession number:
BC006432
Gene ID (NCBI):
857
Gene symbol
CAV1
Synonyms

Background

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).


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