|Positive WB detected in||A549 cells, HeLa cells|
|Positive IHC detected in||human skin cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66067-1-Ig is Caveolin-1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Caveolin-1 fusion protein Ag8049|
|Full Name||caveolin 1, caveolae protein, 22kDa|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||20-25 kDa|
|GenBank accession number||BC006432|
|Gene ID (NCBI)||857|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).
Cell Death Dis
The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine.
Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.