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Caveolin-1 Antibody

Caveolin-1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6C2B2

Cat no : 66067-1-Ig

Synonyms



Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66067-1-Ig is Caveolin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caveolin-1 fusion protein Ag8049
Full Name caveolin 1, caveolae protein, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC006432
Gene symbol CAV1
Gene ID (NCBI) 857
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Protocols

Product Specific Protocols
WB protocol for Caveolin-1 antibody 66067-1-IgDownload protocol
IHC protocol for Caveolin-1 antibody 66067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Dis

The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine.

Authors - Yu-De Chu
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang