Caveolin-1 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caveolin-1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

6C2B2

Cat no : 66067-1-Ig

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 66067-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66067-1-Ig

  WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells.

• 

  IHC staining of human skin cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66067-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human skin cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 66067-1-Ig is Caveolin-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caveolin-1 fusion protein Ag8049
• Full Name: caveolin 1, caveolae protein, 22kDa
• Calculated molecular weight: 22 kDa
• Observed molecular weight: 20-25 kDa
• GenBank accession number: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Publications

Species	Application	Title
human	IHC	Cell Death Dis; The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine.; Authors - Yu-De Chu
human	WB	Int J Clin Exp Pathol; MicroRNA-451a targets caveolin-1 in stomach cancer cells.; Authors - Yi Wang
human	WB	Proteomics; Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.; Authors - Jing Zhang
mouse	IF	Food Chem Toxicol; α-Hemolysin suppresses osteogenesis by inducing lipid rafts accumulation in bone marrow stromal cells.; Authors - Guanqiao Liu