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CoraLite®594-conjugated Caveolin-1 Monoclonal antibody
Caveolin-1 Monoclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
6C2B2
Cat no : CL594-66067
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human skin cancer tissue, human liver cancer tissue |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
CL594-66067 targets Caveolin-1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Caveolin-1 fusion protein Ag8049 |
Full Name | caveolin 1, caveolae protein, 22kDa |
Calculated Molecular Weight | 22 kDa |
Observed Molecular Weight | 20-25 kDa |
GenBank Accession Number | BC006432 |
Gene Symbol | CAV1 |
Gene ID (NCBI) | 857 |
RRID | AB_2883501 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric cancer (PMID: 25339030).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Caveolin-1 antibody CL594-66067 | Download protocol |
FC protocol for CL594 Caveolin-1 antibody CL594-66067 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mutat Res Downregulated CAV-1 in mouse spinal cord may alleviate bone cancer pain by inhibiting the ERK/CREB pathway |