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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Caveolin-1 Monoclonal antibody

Caveolin-1 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6C2B2

Cat no : CL488-66067

Synonyms

Caveolin 1, CAV1, CGL3, CAV, BSCL3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human skin cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human skin cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human liver cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Caveolin-1 antibody (CL488-66067, Clone: 6C2B2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human liver cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human liver cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human liver cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    IF Staining of human liver cancer using CL488-66067

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Caveolin-1 Monoclona (CL488-66067)

    FC experiment of HeLa using CL488-66067

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caveolin-1 (CL488-66067, Clone:6C2B2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF-P detected inhuman liver cancer tissue, human skin cancer tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL488-66067 targets Caveolin-1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caveolin-1 fusion protein Ag8049
Full Name caveolin 1, caveolae protein, 22kDa
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC006432
Gene Symbol CAV1
Gene ID (NCBI) 857
RRIDAB_2883249
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Caveolin-1 antibody CL488-66067Download protocol
FC protocol for CL Plus 488 Caveolin-1 antibody CL488-66067Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nature

Glycosphingolipid synthesis mediates immune evasion in KRAS-driven cancer

Authors - Mariluz Soula
View Article