CoraLite® Plus 488-conjugated Caveolin-1 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caveolin-1 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6C2B2

Cat no : CL488-66067

Synonyms

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21

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Validation Data Gallery

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  IF Staining of human skin cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  FC experiment of HeLa using CL488-66067

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caveolin-1 (CL488-66067, Clone:6C2B2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human skin cancer tissue, human liver cancer tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66067 is Caveolin-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caveolin-1 fusion protein Ag8049
• Full Name: caveolin 1, caveolae protein, 22kDa
• Calculated molecular weight: 22 kDa
• Observed molecular weight: 20-25 kDa
• GenBank accession number: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C