CBFB Monoclonal antibody, PBS Only

• 

  WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67885-1-Ig (same clone as 67885-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

CBFB Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 67885-1-PBS

Clone No.1D7F2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta

---

Product Information

67885-1-PBS targets CBFB in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31183

  Product name: Recombinant human CBFB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-182 aa of BC018509

  Sequence: MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP

• Full Name: core-binding factor, beta subunit
• Calculated Molecular Weight: 182 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC018509
• Gene Symbol: CBFB
• Gene ID (NCBI): 865
• RRID: AB_2918642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13951
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.