CCBE1 Polyclonal antibody

CCBE1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24449-1-AP

Synonyms

CCBE1, Full of fluid protein homolog, KIAA1983

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Validation Data Gallery

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  IHC staining of human kidney using 24449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24449-1-AP (CCBE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 24449-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24449-1-AP (CCBE1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24449-1-AP is CCBE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCBE1 fusion protein Ag19686
• Full Name: collagen and calcium binding EGF domains 1
• Calculated Molecular Weight: 406 aa, 44 kDa
• GenBank Accession Number: BC046645
• Gene Symbol: CCBE1
• Gene ID (NCBI): 147372
• RRID: AB_2879553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCBE1 (collagen and calcium-binding EGF domain-containing protein 1) is a secreted protein that is indispensible for lymphangiogenesis during development. CCBE1 may be a guidance molecule that regulates lymphangioblast budding and possibly migration (PMID: 19287381). The gene of CCBE1 maps to 18q21.32 and encodes a protein of 44 kDa that contains an EGF-like domain and two collagen-like domains. Mutations in the CCBE1 gene cause Hennekam lymphangiectasia-lymphedema syndrome (PMID: 19935664).