Tested Applications
Positive WB detected in | rat testis tissue |
Positive IP detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25822-1-AP targets CCDC33 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CCDC33 fusion protein Ag22893 Predict reactive species |
Full Name | coiled-coil domain containing 33 |
Calculated Molecular Weight | 958 aa, 107 kDa |
Observed Molecular Weight | 85 kDa |
GenBank Accession Number | BC025689 |
Gene Symbol | CCDC33 |
Gene ID (NCBI) | 80125 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N5R6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The human CCDC33 gene was reported to encode a cancer/testis (CT) antigen, which is located on 15q24.1, spans a region of 99.8 kb, and consists of 20 exons (PMID: 28639886). CCDC33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 5 different transcripts (37kda, 41kda, 85kda, 107kda, 114kda). Human CCDC33 was reported to be a cancer/testis (CT) protein. Abundant expression of Ccdc33 is evident during mouse spermatogenesis (PMID: 33942254).
Protocols
Product Specific Protocols | |
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WB protocol for CCDC33 antibody 25822-1-AP | Download protocol |
IP protocol for CCDC33 antibody 25822-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |